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Beta-lactam antibiotics are among the most widely used antibiotics for the treatment of bacterial infections in animals. Beta-lactams are also administered to animals in feed for growth promotion and for collective prophylactic treatment. The monitoring of Beta-lactam residues in milk is important because of the hypersensitivity of some individuals to these antibiotics and also because of the emergence of antibiotic-resistant strains of bacteria. In addition, residual level of Beta-lactams can alter the efficiency of industrial processing of raw milk toward cheese or other fermented dairy products preparation. Maximum residue limits have been specified for food products and milk to control the levels of these antibiotics reaching the consumer.